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Comparison of the Effect of Breast Tumor Tissue Proteome on PI3K/AKT and Ras/ERK Signaling Pathways Activity in Luminal Patients with Higher and Lower than 30 BMI

Introduction: Studies have found that overactivity of mitogenic signaling pathways and suppression of tumor suppressors’ expression play the most important role in breast cancer development and pathogenesis. In this study, we compare the breast tissue proteome of women with a BMI higher than 30 with those with a BMI lower than 30.

Methods: In this descriptive-analytical study, tumor tissue biopsy was done on 10 women diagnosed with invasive ductal breast cancer. Subjects were grouped into two groups, high BMI (33.41 on average) and low BMI (26.04 on average). Proteomics analysis was done on homogenized proteins of the breast tumor biopsy. Data analysis was done using ImageMaster 2D Platinum 6.0 and t-test with 95% confidence interval. Protein identification was done using MALDI-TOF MS/MS mass spectrophotometry.

Results and Discussion: Glyceraldehyde 3-phosphate, hemoglobin alpha chain and calreticulin expression levels were higher in high BMI group in comparison to low BMI group (P<0.05). On the contrary, cytokratin 19, collagen (I) alpha-1 chain and superoxide dismutase expression levels were lower in in high BMI group in comparison to low BMI group (P<0.05). Apo A-1 protein and albumin were only expressed in low BMI group and constant region of the immunoglobulin gamma chain, galectin-1 and HSP27 were exclusively expressed in high BMI group.

Conclusion: Among the wide range of protein expression changes, the changes of several proteins mentioned in the results may affect the metabolic changes in obese individuals, which requires further studies.


Masoumeh Hosseini

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