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Abstract

Apoptotic and Anti-Metastatic Effect of Organotin Coordination Polymer on Human Hepatocellular Carcinoma (Hepg2) Cells via Intrinsic Pathway of Apoptosis

Objective: Dysregulated activation of cellular signaling pathways was shown to be involved in hepatocellular carcinoma (HCC) progression This study focused on the investigation of the anticarcinogen activity of a tin (I) 3-Dsupramolecular coordination polymer (OSCP), a novel organotin supramolecular coordination polymers (SCP namely, 3∞[Ph3SnCu(CN)2.(3-mpy)2] 3∞[Ph3SnCu(CN)2. (3-mpy)2] on HepG2.

Design and Methods:This inhibitory effect was confirmed by cell proliferation assay, cell morphology examination, cell adhesion assay and DNA fragmentation. We have investigated the effects of OSCP on E-cadherin, β-catenin, Bcl-2, and Bax expression by both real time PCR and western blot analysis.

Results:The restrictive effect of OSCP compound on HepG2 cells’ proliferation was evaluated by MTT assay. We observed that treated cells for 24 h with OSCP induced cell death in a dose-dependent manner. Furthermore, Western blot and real time PCR analysis revealed that Bcl-2 and β-catenin protein expression was inhibited after 24 h of treatment with OSCP, while Ecadherin and Bax expression increased after treatment, so OSCP can effectively inhibit the invasive potential of HepG2 cells by altering apoptosis and via inhibition of Bcl-2 and beta catenin which may play a significant role in this process.

Conclusion:These results confirmed the potential role of OSCP as an anticancerigen agent in hepatocarcinoma cell lines.


Author(s):

Mohammad Ibrahim Alallah, Noura M Darwish, Mohamed Soliman Elshikh and M. Ajmal Ali



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